Résumé
Summary
PhD level scientist seeking transition from academic research into a career in a biotechnology company. I have over nine years of experience working in teams and international collaborations in academic research, employing a wide range of techniques in fields including cellular and molecular biology, microbiology, genetics, biochemistry, and host-microbe interactions.
- Experienced in assay design and development, snRNAseq, organoid culture, cell culture, RT-PCR and RNAseq
- Authored original scientific publications in peer reviewed journals. Presented work at international conferences: oral and interpersonal communications
- Demonstrated autonomy from graduate student to postdoctoral scholar.
- Demonstrated ability to set goals, project management and meet deadlines
Education
Ph.D., Cellular and Molecular Biology, University of São Paulo, Brazil, 2021. Thesis: Functional characterization of outer membrane vesicles from the opportunistic pathogen Chromobacterium violaceum
M.Sc., Cellular and Molecular Biology, University of São Paulo, Brazil, 2017. Dissertation: Characterization of eukaryotic-like serine/threonine kinases of Chromobacterium violaceum
B.Sc., Biological Sciences, Federal University of São Carlos, Brazil, 2015.
Research Experience
Department of Infectious Diseases, Boston Children’s Hospital | Harvard Medical School 2022-present
Postdoctoral Research Fellow – Focused on understanding the molecular mechanism of the interactions between the microbiota and intestinal homeostasis
- Discovered a new pathway that regulates cell differentiation and homeostasis in the gut.
- Collaborated with a project that described the mechanism in which a bacterial pathogen evades the intestinal innate immunity.
Department of Cellular and Molecular Biology, University of São Paulo 2017-2021
Doctoral researcher – Focused on bacterial interspecies interactions and pathogenesis
- Discovered a mechanism by which bacteria delivers antibiotics during competition against other species.
- Described a new mechanism that bacterial pathogens use to secrete membrane vesicles during infection to promote adaptation and pathogenesis.
Department of Microbiology, University of North Carolina at Chapel Hill 2019-2020
Visiting researcher award – Focused on innate immunity response against bacterial pathogens
- Discovered novel regulation of inflammasome activation in macrophages by membrane vesicles.
Department of Cellular and Molecular Biology, University of São Paulo 2015-2017
Master researcher – Focused on the characterization of virulence factors from an opportunistic pathogen
- Identified and characterized proteins important during pathogenesis.
- Mentored undergraduate students in the disciplines of cellular and molecular biology.
Technical Expertise
Molecular Biology
- DNA/RNA isolation and quantification from:
- bacteria
- mouse
- human cells/tissues
- Cloning
- PCR, qPCR, RT-PCR
- Gene expression analysis by RNA-seq
- snRNAseq
Microbiology and Genetics
- Vector construction
- Strain generation
- Gene deletions and insertions
- Optimization of microbial growth
- Antibiotic resistance screening assays
Cell Biology
- primary and immortalized cell culture
- Organoids
- Confocal microscopy imaging and analysis
- Transmission and scanning electron microscopy imaging
Protein Biochemistry and Characterization
- Polypeptide synthesis using E. coli system
- Polypeptide isolation and characterization
- Polypeptide over-expression, purification, and quantification
- Proteomic analysis
- Affinity chromatography
- SDS PAGE
- Western blotting
- Preparative HPLC
- Protein-protein interaction assay
Biochemical and Immunology Assays
- β-galactosidase assays using 96-well plate reader
- Polyclonal antibody production
- ELISA
- Cytotoxicity assays
In Vivo Procedures
- Mice husbandry, infection, organ removal and processing
Volunteer Experience
Ad Hoc Reviewer
- Journal of Applied Microbiology 2022-present
Conference Organization
- Symposium on Bacterial Pathogenesis and Host Response, São Paulo, Brazil, 2019
- Symposium in Cell and Molecular Biology, São Paulo, Brazil, 2018
- XV Summer Course in Cellular and Molecular Biology, São Paulo, Brazil, 2018
- XIII Summer Course in Cellular and Molecular Biology, São Paulo, Brazil, 2016
Teaching and Mentoring Experience
Postdoctoral Research Fellow at Boston Children’s Hospital | HMS
2022-Present
- Mentored research assistants and technicians
Tutor and Lab supervisor at University of São Paulo, Brazil
2018-2020
- Conduced and supervised laboratory sessions for medical undergraduate courses of Cellular and Molecular Biology, and Practice in Parasitology
- Instructed students during lab sessions, weekly discussions, and exam review sessions
- Trained, supervised, and managed summer interns as a graduate researcher
Publications
B. E. Jugder, J. H. Batista, J. A. Gibson, P. M. Cunningham, J. M. Asara, P.I. Watnick. Vibrio cholerae high cell density quorum sensing activates de host intestinal innate immune response. 2022. Cell Reports. doi: 10.1016/j.celrep.2022.111368
Batista, J. H., Leal, F. C., Fukuda, T. T. H., Diniz, J. A., Almeida, F. B. R., Pupo, M. T. and da Silva Neto, J. F. Interplay between two quorum sensing-regulated pathways, violacein biosynthesis and VacJ/Yrb, dictates outer membrane vesicle biogenesis in Chromobacterium violaceum. 2020. Environmental Microbiology. doi: 10.1111/1462- 2920.15033
Barroso, K. C. M., Previato-Mello, M., Batista, B. B., Batista, J. H. and da Silva Neto, J. F. EmrR-Dependent Upregulation of the Efflux Pump EmrCAB Contributes to Antibiotic Resistance in Chromobacterium violaceum. 2018. Frontiers in Microbiology. doi: 10.3389/fmicb.2018.02756
Batista, J. H. and da Silva Neto, J. F. Chromobacterium violaceum Pathogenicity: Updates and Insights from Genome Sequencing of Novel Chromobacterium Species. 2017. Frontiers in Microbiology. doi: 10.3389/fmicb.2017.02213
Oral Presentations
64th Annual Drosophila Research Conference, workshop in Immunemetabolism. The Vibrio cholerae quorum sesing regulator, HapR, promotes Drosophila survival by preventing intestinal serotonin depletion. Chicago, 2023
XI Symposium in Cell and Molecular Biology. Functional characterization of outer membrane vesicles from the opportunistic pathogen Chromobacterium violaceum. São Paulo, Brazil, 2019
VIII Symposium in Cell and Molecular Biology. Characterization of eukaryotic-like serine/threonine kinases of Chromobacterium violaceum. São Paulo, Brazil, 2016